Simultaneous stereoselective high-performance liquid chromatographic determination of 10-hydroxycarbazepine and its metabolite carbamazepine-10,11-trans-dihydrodiol in human urine
A. Volosov et al., Simultaneous stereoselective high-performance liquid chromatographic determination of 10-hydroxycarbazepine and its metabolite carbamazepine-10,11-trans-dihydrodiol in human urine, J CHROMAT B, 738(2), 2000, pp. 419-425
An enantioselective HPLC method for the simultaneous determination of the c
oncentration of the enantiomers of the oxcarbazepine metabolites 10-hydroxy
carbazepine (MHD) and carbamazepine-10,11-trans-dihydrodiol (DHD) in human
urine is described. The method is based on extraction with tert.-butylmethy
l ether-dichloromethane (2:1, v/v) under alkaline conditions, separation an
d evaporation of the organic phase and dissolution of the residue in the mo
bile phase. Enantiomers are resolved on a Diacel Chiralcel OD column (250 m
mx4.6 mm I.D.) under isocratic conditions using as mobile phase n-hexane-et
hanol-2-propanol (18:2:1, v/v/v) with addition of glacial acetic acid (0.1%
), The enantiomers are detected by UV at 215 nm. The method allows reliable
determination of the MHD and DHD enantiomers in human urine with limits of
quantification of 0.2 mg/l and 0.4 mg/l, respectively. (C) 2000 Elsevier S
cience B.V. All rights reserved.