T. Velpandian et al., Development and validation of a new high-performance liquid chromatographic estimation method of meloxicam in biological samples, J CHROMAT B, 738(2), 2000, pp. 431-436
A simple, HPLC method was developed to estimate meloxicam (COX-2 inhibitor)
using piroxicam as the internal standard. The mobile phase containing meth
anol, acetonitrile and an aqueous solution of diammonium hydrogenorthophosp
hate (50 mM) in the ratio of 4:1:5 was pumped at the rate 1 ml/min. Lichroc
art RP-18 (125x4 mm) was used as an analytical column and the analytes were
detected at 364 nm using a UV detector. Acidified plasma samples were extr
acted with chloroform, evaporated to dryness, reconstituted in the mobile p
hase and then a volume of 10 mu l of the prepared sample was injected in th
e column. The retention time of meloxicam and piroxicam was found to be 2.7
and 1.9, respectively. This method showed an accuracy of 102.3% at 0.52 mu
g/ml and was capable of detecting a minimum concentration of 0.029 mu g/ml
meloxicam from biological samples. The analytical method was successfully
utilized for estimating meloxicam in biological samples. (C) 2000 Elsevier
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