Validated method for the therapeutic drug monitoring of flunitrazepam in human serum using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry with an ion trap detector
J. Darius et P. Banditt, Validated method for the therapeutic drug monitoring of flunitrazepam in human serum using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry with an ion trap detector, J CHROMAT B, 738(2), 2000, pp. 437-441
An HPLC-MS-MS method for the quantitative analysis of flunitrazepam in huma
n serum was established. The method features a very simple liquid-liquid ex
traction, the use of a standard 4-mm HPLC column, isocratic elution using a
buffer-free solvent, short retention times in connection with good peak re
solution and the sensitivity and selectivity of an ion trap MS-MS detector.
The procedure enables unambiguous identification of analytes by their prod
uct ion spectra, as well as sensitive quantitation (limit of quantitation f
or flunitrazepam=0.5 ng/ml). This feature was used for the confirmation of
HPLC-UV results for nitrazepam. (C) 2000 Elsevier Science B.V. All rights r
eserved.