Sm. Barratt-boyes et al., Maturation and trafficking of monocyte-derived dendritic cells in monkeys:Implications for dendritic cell-based vaccines, J IMMUNOL, 164(5), 2000, pp. 2487-2495
Human dendritic cells (DC) have polarized responses to chemokines as a func
tion of maturation state, but the effect of maturation on DC trafficking in
vivo is not known, We have addressed this question in a highly relevant rh
esus macaque model. We demonstrate that immature and CD40 ligand-matured mo
nocyte-derived DC have characteristic phenotypic and functional differences
in vitro. In particular, immature DC express CC chemokine receptor 5 (CCR5
) and migrate in response to macrophage inflammatory protein-1 alpha (MIP-1
alpha), whereas mature DC switch expression to CCR7 and respond exclusivel
y to MIP-3 beta and 6Ckine, Mature DC transduced to express a marker gene l
ocalized to lymph nodes after intradermal injection, constituting 1.5% of l
ymph node DC. In contrast, cutaneous DC transfected in situ via gene gun we
re detected in the draining lymph node at a 20-fold lower frequency. Unexpe
ctedly, the state of maturation at the time of injection had no influence o
n the proportion of DC that localized to draining lymph nodes,as labeled im
mature and mature DC were detected in equal numbers; Immature DC that traff
icked to lymph nodes underwent a significant up-regulation of CD86 expressi
on indicative of spontaneous maturation, Moreover, immature DC exited compl
etely from the dermis within 36 h of injection, whereas mature DC persisted
in large numbers associated with a marked inflammatory infiltrate, We conc
lude that in vitro maturation is not a requirement for effective migration
of DC in vivo and suggest that administration of Ag-loaded immature DC that
undergo natural maturation following injection may be preferred for DC-bas
ed immunotherapy.