Mj. Wiselka et al., Cytomegalovirus viraemia has poor predictive value for the development of cytomegalovirus disease in patients with advanced HIV-infection, J INFECTION, 39(3), 1999, pp. 187-192
Objective: Cytomegalovirus (CMV) continues to be one of the most important
opportunistic infections associated with human immunodeficiency virus (HIV)
infection, This study investigated the value of CMV-viraemia in predicting
the development of clinical CMV disease in patients with advanced HIV infe
ction.
Methods: This was a prospective observational study performed over a 2-year
period between 1994-96 in the Department of Infection and Tropical Medicin
e at Leicester Royal Infirmary, Adult HIV-positive patients attending a hos
pital clinic were included if they were CMV-seropositive with CD4 counts le
ss than or equal to 50 cells/mm(3), Subjects were seen at approximately 6-w
eekly intervals in the clinic and were reviewed by an experienced ophthalmo
logist, Serum for CMV PCR was taken and stored at regular intervals and qua
litative and quantitative PCR was performed at the end of the study period.
The value of PCR in predicting the development of CMV disease was then ass
essed.
Results: Twenty-six patients were followed up during the study period and 7
7 evaluable specimens were analysed for CMV PCR. Twenty-three (30%) samples
were positive and 54 negative. Seven (27%) patients developed CMV disease
(five retinitis alone, and two with retinitis and oesophagitis) during the
study period. Viraemia was often intermittent and there was no significant
difference in the proportions of patients with positive or negative tests w
ho subsequently developed CMV disease, The sensitivity, specificity, positi
ve and negative predictive values of the qualitative PCR were 71%, 47%, 33%
, and 82% respectively and 57%, 74%, 44% and 82% respectively for the quant
itative PCR (>10(3) copies:ml),
Conclusions: The results from this study, which was performed before the in
troduction of protease inhibitors, found that cytomegalovirus PCR was of li
mited clinical value in predicting the patients at greatest risk; of develo
ping CMV-disease and provided little useful prognostic information. (C) 199
9 The British Infection Society.