Polarized distribution of interleukin-1 receptors and their role in regulation of serotonin transporter in placenta

We investigated the expression of interleukin-1 (IL-1) receptors and their involvement in the regulation of the serotonin transporter gene expression in human placenta. IL-1 beta is an activator of the serotonin transporter g ene expression in JAR human placental choriocarcinoma cells as demonstrated by an increase in the steady-state levels of the transporter mRNA and in s erotonin transport activity. This activation is blocked by IL-1 receptor an tagonist. Genistein also blocks the effect of IL-1 beta, indicating involve ment of tyrosine phosphorylation in the process. Treatment of JAR cells wit h IL-1 beta activates mitogen-activated protein kinases and nuclear factor- kappa B. The nuclear factor-kappa B that is responsive to IL-1 beta in thes e cells is the p65 homodimer. Northern blot analysis and reverse transcript ion-polymerase chain reaction revealed that JAR cells and human placenta ex press type I and type II IL-1 receptors. The binding sites for I-125-IL-1 b eta are localized predominantly in the maternal-facing brush border membran e of the syncytiotrophoblast. These results show that IL-1 in the maternal circulation is likely to play a critical role in the regulation of the sero tonin transporter gene expression in the placenta.