Inactivation of bovine herpesvirus 1 in semen using a hyperimmune egg yolksemen extender

Authors
Silva, N Solana, A Castro, JM
Citation
N. Silva et al., Inactivation of bovine herpesvirus 1 in semen using a hyperimmune egg yolksemen extender, J VET MED B, 47(1), 2000, pp. 69-75
Citations number
26
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARYPUBLIC HEALTH
ISSN journal
09311793 → ACNP
Volume
47
Issue
1
Year of publication
2000
Pages
69 - 75
Database
ISI
SICI code
0931-1793(200002)47:1<69:IOBH1I>2.0.ZU;2-U
Abstract
Hyperimmune egg yolk semen extender was used for the inactivation of bovine herpesvirus (BHV-1) in experimentally infected bovine semen. As much as 5 x 10(4) TCID50/ml of virus was inactivated in semen as assayed by tissue cu lture. Moreover the hyperimmune egg yolk semen extender did not produce any adverse effect on the quality of the semen after being frozen/thawed in co mparison with normal egg yolk semen extender (P > 0.05). The hyperimmune eg g yolk semen extender is considered an important tool for containing the sp read of BHV-1 from infected semen.