Recombinant adeno-associated virus expressing human papillomavirus type 16E7 peptide DNA fused with heat shock protein DNA as a potential vaccine for cervical cancer
Dw. Liu et al., Recombinant adeno-associated virus expressing human papillomavirus type 16E7 peptide DNA fused with heat shock protein DNA as a potential vaccine for cervical cancer, J VIROLOGY, 74(6), 2000, pp. 2888-2894
In this study, we explore a potential vaccine for human papillomavirus (HPV
)-induced tumors, using heat shock protein as an adjuvant, a peptide vaccin
e for safety, and adeno-associated virus (AAV) as a gene delivery vector. T
he tumor vaccine was devised by constructing a chimeric gene which containe
d HPV type 16 E7 cytotoxic T-lymphocyte (CTL) epitope DNA (M. C. Feltkamp,
H, L. Smits, M. P. Vierboom, R. P. Minnaar, B. M. de Jongh, J. W. Drijfhout
, J. ter Schegget, C. J. Melief, and W. RI. Kast, fur. J, Immunol, 23:2242-
2249, 1993) fused with the heat shock protein gene as a tumor vaccine deliv
ered via AAV. Our results demonstrate that this vaccine can eliminate tumor
cells in syngeneic animals and induce CD4- and CD8-dependent CTL activity
in vitro. Moreover, studies with knockout mice with distinct T-cell deficie
ncies confirm that CTL-induced tumor protection is CD4 and CD8 dependent. T
aken together, the evidence indicates that this chimeric gene delivered by
AAV has potential as a cervical cancer vaccine.