Neuropeptides and capsaicin stimulate the release of inflammatory cytokines in a human bronchial epithelial cell line

The role of neuropeptides in initiating and modulating airway inflammation was examined in a human bronchial epithelial cell line (i.e. BEAS-2B). At a range of concentrations, exposure of BEAS-2B cells to Substance P (SP) or calcitonin gene related protein resulted in immediate increases in intracel lular calcium ([Ca2+](i)), the synthesis of the transcripts for the inflamm atory cytokines, IL-6, IL-8 and TNF alpha after 2 h exposure, and the relea se of their proteins after 6 h exposure. Addition of thiorphan (100 nM), an inhibitor of neutral endopeptidase, enhanced the levels of SP-stimulated c ytokine release. Stimulation of IL-6 by SP occurred in a conventional recep tor-mediated manner as demonstrated by its differential release by fragment s SP 4-11 and SP 1-4 and by the blockage of IL-6 release with the non-pepti de, NK-I receptor antagonist, CP-99 994. In addition to the direct stimulat ion of inflammatory cytokines, SP (0.5 mu M), in combination with TNF alpha (25 units/ml), synergistically stimulated IL-6 release. BEAS-2B cells also responded to the botanical irritant, capsaicin (10 mu M) with increases in [Ca2+](i) and IL-8 cytokine release after 4 h exposure. The IL-8 release w as dependent on the presence of extracellular calcium. Capsaicin-stimulated increases of [Ca2+](i) and cytokine release could be reduced to control le vels by pre-exposure to capsazepine, an antagonist of capsaicin (i.e. vanil loid) receptor(s) or by deletion of extracellular calcium from the exposure media. The present data indicate that the BEAS-2B human epithelial cell li ne expresses neuropeptide and capsaicin-sensitive pathways, whose activatio n results in immediate increases of [Ca2+](i) stimulation of inflammatory c ytokine transcripts and the release of their cytokine proteins. (C) 1999 Ha rcourt Publishers Ltd.