Genetic marker analysis was performed on 98 Posavina horse and 16 Trak
ehner horse blood samples from Croatian populations. All samples were
tested for genetic types at seven red blood cell alloantigen (blood gr
oup) loci and ten biochemical genetic loci. The results were analyzed
to determine genetic variability levels within the breeds and to exami
ne genetic relationships of the horse breeds from Croatia with other b
reeds of domestic horses. Genetic variability within the Trakehner hor
se was relatively low compared to the mean for domestic horses. Mean u
nbiased expected heterozygosity for 87 populations of domestic breeds
was 0.442 (STD = 0.045) compared to 0.398 for the Trakehner horse form
Croatia. In contrast, genetic variation in the Posavina horse was rel
atively high with an unbiassed expected heterozygosity of 0.495. Compa
risons of observed and expected heterozygosity within both breeds show
ed no evidence of inbreeding. Here we reported on gene marker frequenc
ies for the Posavina horse and Trakehner horse from Croatia and geneti
c resemblance of these horse breeds to other domestic breeds as estima
ted by restricted maximum likelihood techniques.