COMPARISON OF ENZYMATIC TECHNIQUE AND MOB ILE BAG TECHNIQUE FOR DETERMINATION OF INTESTINAL DIGESTIBILITY OF FEEDSTUFF UNDEGRADABLE PROTEIN

The goal of this study was to compare the values of intestinal digesti bility of undegraded crude protein in the rumen of ruminants, determin ed by a mobile bag technique in cannulated animals and by an enzymatic technique using pancreatin, for a wider range of feedstuffs. The basi c set of feedstuffs was divided into subsets of bulk feeds, concentrat es and extracted meals with feedstuffs from animal sources for better specification of predicted values of intestinal digestibility. The rel ation of values determined by a mobile bag technique to Values determi ned by an enzymatic technique with pancreatin was expressed by equatio ns of linear regression for the separate subsets of feedstuffs. Tab. I shows parameters of equations for all sets while the relation between values of intestinal digestibility of undegraded crude protein in the rumen obtained by a mobile bag technique and by an enzymatic techniqu e is shown in Figs. 1-3. The largest subset of bulk feeds (n = 39), wh ich included green forage of alfalfa, clover, meadow grass, clover-gra ss, meadow foxtail and timothy, silage of corn, beet tops, alfalfa, cl over, crushed beans, barley and pea with wheat, grass silage with high er dry matter content, and alfalfa hay and barley straw, had the lowes t value of correlation coefficient (r = 0.760, %RSD = 7.69). The highe st value of correlation coefficient was recorded for the subset of ext racted meals (cottonseed, rapeseed, soybean, linseed, groundnuts and s esame) with feedstuffs from animal sources (fish and meat-bone meals a nd blood meal) - r = 0.941, %RSD = 3.48, n = 14. The third subset, con sisting of grains (n = 22), i.e. wheat, barley, rye, oats, corn, had t he correlation coefficient r = 0.852 and %RSD = 5.05.