Lung injury and surfactant metabolism after hyperventilation of premature lambs

We asked whether lung injury and surfactant metabolism differed in preterm lambs after a 1-h period of hyperventilation to PCO2 values of 25-30 mm Hg. The lambs then were surfactant treated and conventionally ventilated (CV) or high-frequency oscillatory ventilated (HFOV) for an additional 1 or 8 h. The results were compared with lambs that were not hyperventilated or surf actant treated but were ventilated with CV or HFOV. The 1-h hyperventilatio n resulted in increased alveolar protein, increased recovery of intravascul ar [I-131]albumin in the lungs, and an increase in tumor necrosis factor-al pha mRNA. Then were no differences between CV or HFOV in alveolar or total lung recoveries of saturated phosphatidylcholine (Sat PC), tracer doses of [C-14]Sat PC and [I-125]surfactant protein-B, or in percent Sat PC in large aggregate surfactant in surfactant-treated lambs. The lambs not hyperventi lated or treated with surfactant had lower large aggregate pools and lower recoveries of [C-14]Sat PC and [C-14]surfactant protein-B in total lungs th an for the surfactant-treated lungs, but there were no differences between the CV and HFOV groups. Hyperventilation followed by surfactant treatment r esulted in a mild injury, but the subsequent use of CV or HFOV did not resu lt in differences in surfactant metabolism.