N. Menkovic et al., Quantitative determination of secoiridoid and gamma-pyrone compounds in Gentiana lutea cultured in vitro, PLANTA MED, 66(1), 2000, pp. 96-98
The production of secondary metabolites was studied in shoots, roots, and h
airy roots of Gentiana lutea obtained in vitro. In shoots, both secoiridoid
and gamma-pyrone compounds were detected in amounts similar to those found
in aerial parts of plants collected from nature. The most abundant secoiri
doid was gentiopicrin while mangiferin was the main compound among the gamm
a-pyrones. The adventitious roots obtained in vitro showed a poor biosynthe
tic capacity. Upon infection with Agrobacterium rhizogenes, nine hairy root
clones were established which differed in the amount of secondary metaboli
tes.