Peptide YY stimulates the expression of apolipoprotein A-IV gene in Caco-2intestinal cells

The effect of peptide YY, a gastrointestinal hormone, on the expression of the apolipoprotein A-IV gene in the intestinal epithelial cell line Caco-2 was examined by semiquantitative RT-PCR followed by Southern hybridization with an inner oligonucleotide probe. Apolipoprotein A-IV mRNA levels were i ncreased in response to peptide YY in a dose- and time-dependent fashion, W estern blotting revealed that the exogenous peptide YY increased the intrac ellular concentration of apolipoprotein A-IV, in contrast, apolipoprotein A -I, B, and C-III mRNA did not respond to peptide YY. Differentiated Caco-2 cells expressed Y1- but not Y2- and YS-receptor subtype mRNA, The present r esults suggest that peptide YY modulates apolipoprotein A-IV gene expressio n, likely via the Y1-receptor subtype in intestinal epithelial cells.