Smoking is thought to be one of the most important anthropogenic risk facto
rs involved in the development of urinary bladder cancer in humans. Tobacco
smoke contains a complex mixture of chemicals including potent carcinogens
such as aromatic amines. In the present study the amounts of several freeb
ase aromatic amines including the potent carcinogens 2-aminonaphthalene and
4-aminobiphenyl have been analyzed in the urine of 48 German urban living
smokers and non-smokers. The results indicate that (i) both groups excrete
the identical set of four aromatic amines; (ii) smokers excrete approximate
ly twice the total amount of these amines, but similar amounts of 2-aminona
phthalene and 4-aminobiphenyl are found in non-smokers; and (iii) the excre
ted aromatic amines are decomposed in the urine within a few hours thus, ex
plaining why aromatic amines are difficult to detect in this matrix. Their
decomposition could be prevented by adding small amounts of p-toluidine to
the freshly collected urine. Unlike smokers the origin of aromatic amines d
etected in the urine of non-smokers is at present unknown. Based on the cot
inine levels found in the urine of non-smokers environmental tobacco smoke
can be excluded as a major source of aromatic amines. In addition, neither
diesel exhaust-related nitroarenes nor the corresponding amino-derivatives,
to which they may be metabolically converted, were found. The detected uri
nary levels of aromatic amines arising from sources other than tobacco smok
e or diesel exhaust may play a role in the bladder cancer etiology of non-s
mokers. (C) 2000 Elsevier Science B.V. All rights reserved.