The sterol composition of the Caribbean sponge lotrochota birotulata was in
vestigated. Structure of a new ecdysteroid 2 beta,3 beta,14 alpha,20 beta-t
etrahydroxy-22 alpha-(2-hydroxyacetiloxy)-5 beta-colest-7-en-6-one (1) was
assigned on the basis of spectroscopic and chemical evidence and molecular
mechanics calculations. Isolation of the widespread ecdysteroids 2-5 is als
o reported. (C) 2000 Elsevier Science Inc. All rights reserved.