Jm. Kirkwood et al., PHASE IB TRIAL AT PICIBANIL (OK-432) AS AN IMMUNOMODULATOR IN PATIENTS WITH RESECTED HIGH-RISK MELANOMA, Cancer immunology and immunotherapy, 44(3), 1997, pp. 137-149
The microbial immunostimulant OK-432 has been studied intensively in p
reclinical systems and has shown promise as an anticancer agent in tri
als that have been conducted over the past 20 years in Japan. To date,
no systematic dose response evaluation of this agent has defined its
dose-limiting toxicity or immunobiological activity. A phase IA study
has been conducted in 25 patients with metastatic cancer at the Univer
sity of Pittsburgh Cancer Institute Melanoma Center, establishing 30 K
E as the maximal tolerable dosage, on the basis of cutaneous reactions
, Subsequently, 48 patients with resected high-risk melanoma participa
ted in a phase IB study of OK-432. This study has evaluated the immuno
modulatory activity of OK-432 at five dosages ranging from 1 KE to 20
KE, administered ID twice weekly for 3 months. A formal analysis of th
e treated population in comparison to the randomized control group has
been conducted, and profound immunological effects have been defined
in the group of patients treated with OK-432. Patients who participate
d in this trial had a significant depression of OK-432-inducible cytok
ine production (interleukin-1 beta, interferon gamma, and tumor necros
is factor alpha) at baseline. Treatment with OK-432 reversed this defi
cit for interferon gamma (IFN gamma) production in a dose-dependent ma
nner, and mitigated the inhibition for interleukin-l (IL-1) across all
dosage groups. The impact of OK-432 upon other immunological function
s of the treated cohorts is more variable, with durable suppression of
mononuclear cell superoxide production, and in vitro cytotoxicity to
tumor. Immunological characteristics of the entire cohort demonstrate
a strong and significant correlation of elevated blood CD16(+) cell co
unts and natural killer activity with early tumor progression and deat
h due to melanoma. Favorable prognosis is associated with monocyte cap
acity to produce tumor necrosis factor (TNF), and polymorphonuclear le
ukocyte formylmethionyl-leucylphenylalanine-inducible superoxide relea
se. This study reveals several new immunological correlates of tumor p
rogression and lethal outcome in resected highrisk melanoma. It demons
trates that the depressed IL-1, TNF, and IFN gamma release associated
with melanoma may be mitigated by treatment with OK-432. This study ha
s defined treatment and dose response patterns of immunomodulation ass
ociated with one of the most complex immunological agents yet evaluate
d in phase IB trials, in a well-defined population of high-risk patien
ts with resected melanoma.