Jh. Moses et al., A new HLA-A*02 allele, A*0234, detected by polymerase chain reaction usingsequence-specific primers (PCR-SSP), TISSUE ANTI, 55(2), 2000, pp. 175-177
A novel HLA-A*02 allele, A*0234, was identified in a potential unrelated bo
ne marrow donor typed by polymerase chain reaction using sequence-specific
primers (PCR-SSP). Positive reactions obtained upon testing with PCR-SSP di
d not lit any known combination of alleles indicating the possible presence
of a novel allele. Sequencing of clones from this individual revealed the
presence of a novel allele, HLA-A*0234. The sequence of exons 2, 3 and part
of exon 4 showed that A*0234 differed from A*02011 by a single nucleotide
in exon 2 at position 282 (C to G). The nucleotide substitution results in
an amino acid change at residue 70 (Histidine to Glutamine) in the alpha 1
domain.