K. Kolasa et al., Densitometric analysis of G(alpha o) protein subunit levels from postmortem Alzheimer disease hippocampal and prefrontal cortical membranes, ALZ DIS A D, 14(1), 2000, pp. 53-57
An immunoblotting method using prefrontal cortical and hippocampal membrane
s from control and Alzheimer disease postmortem brains was employed to dete
ct three subtypes of G(alpha o) protein. In the membranes from control subj
ects, the density of G(alpha o1) in hippocampus and cortex was the highest,
whereas the density of G(alpha o2) Was the lowest and that of G(alpha o3)
was intermediate. In the Alzheimer disease membranes from hippocampus, the
density of total G(alpha o) and all three subtype forms was not changed sig
nificantly when compared with control values. There were statistically sign
ificant alterations in G(alpha o) in cortical membranes from Alzheimer dise
ase when compared with controls. The density of G(alpha o1) was decreased b
y approximately 85%, density of G(alpha o3) was decreased by approximately
95%, and total G(alpha o) density was decreased by approximately 84% of con
trol value. However, G(alpha o2) density was decreased by approximately 44%
but was found not to be statistically different from controls.