T. Perez-ruiz et al., Determination of proteins in serum by fluorescence quenching of Rose Bengal using the stopped-flow mixing technique, ANALYST, 125(3), 2000, pp. 507-510
The stopped-flow mixing technique was used to develop a very fast, sensitiv
e and accurate method for determining total proteins. The method is based o
n the lower fluorescence of Rose Bengal caused by binding of the dye to the
proteins. The decrease in the fluorescence intensity, measured at 572 nm w
ith excitation 555 nm, was linearly related to protein concentration from 1
.3 to 24.5 mu g ml(-1). The detection limit was 0.3 mu g ml(-1). The method
was satisfactorily applied to the determination of total proteins in diffe
rent serum samples.