Relationship between local cerebral blood flow and metabolism during mild and moderate hypothermia in rats

Citation
T. Frietsch et al., Relationship between local cerebral blood flow and metabolism during mild and moderate hypothermia in rats, ANESTHESIOL, 92(3), 2000, pp. 754-763
Citations number
36
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Journal title
ANESTHESIOLOGY
ISSN journal
00033022 → ACNP
Volume
92
Issue
3
Year of publication
2000
Pages
754 - 763
Database
ISI
SICI code
0003-3022(200003)92:3<754:RBLCBF>2.0.ZU;2-J
Abstract
Background: Hypothermia may interfere with the relationship between cerebra l blood now (CBF) and metabolism. Because this conclusion was based on the analysis of global values. the question remains whether hypothermic CBF/met abolism uncoupling exists on a local cerebral level. This study investigate d the effects of hypothermic anesthesia on local cerebral blood now (LCBF) and local cerebral glucose utilization (LCGU). Methods: Thirty-six rats were anesthetized with isoflurane (1 minimum alveo lar concentration) and artificially ventilated to maintain normal arterial carbon dioxide partial pressure (pH-stat). Pericranial temperature was main tained as normothermic (37.5 degrees C, n = 12) or was reduced to 35 degree s C (n = 12) or 32 degrees C (n = 12), Pericranial temperature was maintain ed constant for 60 min until LCBF or LCGU were measured by autoradiography. Twelve conscious rats served as normothermic controls. Results: Compared with conscious animals, mean CBI: remained unchanged duri ng normothermic anesthesia. Mean CBI: significantly increased during mild h ypothermia but was unchanged during moderate hypothermia. During normotherm ic anesthesia, mean CGU was 45% lower than in conscious controls (P < 0.05) , No further CGU reduction was found during mild hypothermia, whereas CGU f urther decreased during moderate hypothermia (48%; P < 0.05), Local analysi s showed a Linear LCBF/LCGU relationship in conscious (r = 0.94) and anesth etized (r = 0.94) normothermic animals, as well as in both hypothermic grou ps (35 degrees C: r = 0.92; 32 degrees C: r = 0.95; P < 0.05), The LCBF-to- LCGU ratio increased from 1.4 (conscious controls) to 2.4 (normothermic iso flurane) and 3.6 ml/mu mol (mild and moderate hypothermia, P < 0.05), Conclusions: Decrease of mean CGU at unchanged or increased mean CBF during hypothermic anesthesia may not indicate uncoupling. Local analysis shows a maintained linear relationship that is reset to a higher CHF/CGU ratio.