M. Raponi et al., The influence of antisense gene location on target gene suppression in thefission yeast Schizosaccharomyces pombe, ANTISENSE N, 10(1), 2000, pp. 29-34
A fission yeast model was employed to investigate the influence of antisens
e gene location on the efficacy of antisense RNA-mediated target gene suppr
ession. Fission yeast transformants were generated that contained the targe
t lacZ gene at a fixed position and a single copy antisense lacZ gene integ
rated into various genomic locations, including the same locus as the targe
t gene. No significant difference in lacZ suppression was observed when the
antisense gene was integrated in close proximity to the target gene locus
compared with other genomic locations, indicating that target and antisense
gene colocalization is not a critical factor for efficient antisense RNA-m
ediated gene expression in vivo. Instead, increased lacZ downregulation cor
related with an increase in antisense dose, with the steady-state levels of
antisense RNA being dependent on genomic position effects and transgene co
py number.