Citation
A. Pollice et al., In vitro binding of nucleolin to double-stranded telomeric DNA, BIOC BIOP R, 268(3), 2000, pp. 909-915
Citations number
41
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X
→ ACNP
Volume
268
Issue
3
Year of publication
2000
Pages
909 - 915
Database
ISI
SICI code
0006-291X(20000224)268:3<909:IVBONT>2.0.ZU;2-5
Abstract
We have purified a 100 kDa protein, resolved in a Southwestern binding scre
en of total nuclear proteins from Hela cells with double-stranded human tel
omeric probe. A polyclonal antiserum raised by this protein recognizes puri
fied nucleolin and stains nucleoli in growing Hela cells. We demonstrate th
at a truncated form of human nucleolin and a purified deletion derivative o
f mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggest
s a new link between telomeres and the nucleolus. (C) 2000 Academic Press.