A. Puga et al., Activation of transcription factors activator protein-1 and nuclear factor-kappa B by 2,3,7,8-tetrachlorodibenzo-p-dioxin, BIOCH PHARM, 59(8), 2000, pp. 997-1005
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD; dioxin), the prototype agonist o
f the aromatic hydrocarbon (Ah) receptor, is a potent tumor promoter as wel
l as a complete liver carcinogen that produces an oxidative stress response
in rodents and in cultured cell lines. It has been proposed that TCDD prom
otes neoplastic transformation through oxidative signal transduction pathwa
ys, which results in activation of immediate-early response transcription f
actors. To set the stage for a test of this hypothesis, we evaluated the ef
fect of TCDD treatment on the activation of several transcription factors,
including those in the nuclear factor-kappa B (NF-kappa B) and activator pr
otein-1 (AP-1) families, which are activated by changes in the redox state
of cells. In an extension of prior results, we found that TCDD treatment pr
oduced a sustained overexpression of AP-1 for at least 72 hr in wild-type m
ouse hepatoma Hepa-l cells, but not in the Ah receptor-deficient derivative
c35 or in cytochrome P450-1A1 (CYP1A1) negative c37 cells. In addition, TC
DD treatment caused a significant increase in the DNA binding activity of N
F-kappa B, but not in the activities of the other transcription factors tes
ted. AP-1 and NF-kappa B activation were blocked by the thiol antioxidant N
acetylcysteine and by nordihydroguaiaretic acid, an antioxidant and lipoox
ygenase inhibitor and an inhibitor of the epoxygenase activity of CYP1A1, a
nd did not take place in c35, c37, or in Ah nuclear translator-deficient c4
cells. Hence, sustained activation of these two transcription factors by T
CDD is likely to result from a CYP1A1-dependent and Ah receptor complex-dep
endent oxidative signal. Electrophoretic mobility supershift analyses with
specific antibodies showed that most of the increase in NF-kappa B binding
activity could be accounted for by increases in p50/p50 complexes. Since th
ese complexes are known to repress NF-kappa B-dependent gene transcription,
our results delineate a second molecular mechanism, in addition to the rec
ently found block of tumor necrosis factor oc-mediated p50/p65 activation,
that may be responsible for the immunosuppresive effects of TCDD. (C) 2000
Elsevier Science Inc.