Molecular characterization of ubiquitin genes from Aspergillus nidulans: mRNA expression on different stress and growth conditions

We are interested in studying the ubiquitin (UBI) gene expression during di fferent stress and growth conditions in the filamentous fungus Aspelgillus nidulans. Here, we report the cloning of a cDNA clone that corresponds to a gene, ubi1, that encodes a carboxyl extension protein from A. nidulans. Th is cDNA corresponds to a gene that encodes a protein that showed high homol ogy to other polyubiquitin and CEP-80 genes at the N- and C-terminus, respe ctively. We characterize the mRNA expression of the CEP and polyubiquitin g enes during several growth and stress conditions. Expression of the ubi1 an d ubi4 genes was correlated with cell growth in most of the carbon sources used, except maltose. Both ubi1 and ubi4 genes were induced upon heat-shock , although the levels of expression were raised quicker for ubi4 than for u bi1. The ubi1 and ubi4 genes displayed a very complex expression pattern in presence of drugs with a different mechanism of action suggesting that the regulatory processes controlling UBI gene expression discriminate between different stresses and can affect individually each UBI gene. The ubi1 gene was highly expressed in presence of hydrogen peroxide while the ubi4 mRNA level was not affected; several metals in our experimental conditions were not able to induce either ubi1 nor ubi4 genes. (C) 2000 Elsevier Science B. V. All rights reserved.