Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2 '-deoxyuridine-containing oligodeoxynucleotides

Authors
Malakhov, AD Malakhova, EV Kuznitsova, SV Grechishnikova, IV Prokhorenko, IA Skorobogatyi, MV Korshun, VA Berlin, YA
Citation
Ad. Malakhov et al., Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2 '-deoxyuridine-containing oligodeoxynucleotides, BIOORG KHIM, 26(1), 2000, pp. 39-50
Citations number
69
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
26
Issue
1
Year of publication
2000
Pages
39 - 50
Database
ISI
SICI code
0132-3423(200001)26:1<39:SAFPO5>2.0.ZU;2-C
Abstract
Novel reagents for the fluorescent labeling of oligo- and polynucleotides h ave been prepared: 5-(1-pyrenylethynyl)-2'-deoxyuridine 3'-phosphoramidite and a solid support carrying this nucleoside. Oligonucleotides containing o ne or several modified units have been synthesized, and the fluorescence of these probes has been shown to change upon hybridization with the compleme ntary sequence.