Description of a nonlethal herpes simplex virus type 1 glycoprotein D deletion mutant affecting a site frequently used for PCR

Authors
Coyle, PV Jain, S Wyatt, D McCaughey, C O'Neill, HJ
Citation
Pv. Coyle et al., Description of a nonlethal herpes simplex virus type 1 glycoprotein D deletion mutant affecting a site frequently used for PCR, CL DIAG LAB, 7(2), 2000, pp. 322-324
Citations number
12
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
7
Issue
2
Year of publication
2000
Pages
322 - 324
Database
ISI
SICI code
1071-412X(200003)7:2<322:DOANHS>2.0.ZU;2-A
Abstract
We report a herpes simplex virus type 1 mutant which failed to amplify with a commonly used glycoprotein D primer set. The virus contained a nine-base deletion in the gene's 5' nontranslated region. The altered amplicon was c learly distinguishable on a 4% high-resolution agarose gel.