Isolation of bacteria other than Helicobacter pylori from stomachs of squirrel monkeys (Saimiri spp.) with gastritis

Citation
Ss. Khanolkar-gaitonde et al., Isolation of bacteria other than Helicobacter pylori from stomachs of squirrel monkeys (Saimiri spp.) with gastritis, DIG DIS SCI, 45(2), 2000, pp. 272-280
Citations number
18
Categorie Soggetti
Gastroenerology and Hepatology","da verificare
Journal title
DIGESTIVE DISEASES AND SCIENCES
ISSN journal
01632116 → ACNP
Volume
45
Issue
2
Year of publication
2000
Pages
272 - 280
Database
ISI
SICI code
0163-2116(200002)45:2<272:IOBOTH>2.0.ZU;2-M
Abstract
Gastric biopsy specimens obtained from 12 squirrel monkeys (Saimiri spp.) w ere investigated by culture for the presence of bacteria. The stomachs of t wo monkeys with gastritis were colonized with gram-negative, urease-positiv e bacteria, identified as Ochrobactrum anthropi by the Vitek and API NFT me thods (BioMerieux). A third monkey with gastritis was positive for Aeromona s salmonicida and Pseudomonas vesicularis (both urease-negative). No Helico bacter pylori was isolated from squirrel monkeys. Light microscopic and tra nsmission electron microscopic examination revealed that the O. anthropi is olates were covered by extracellular material, indicating a capsule. Charac terization of the O. anthropi urease revealed Michaelis-Menten constants (K -m values) of 6.2 and 4.0 mM urea for the ureases of O. anthropi isolates S 664 and S1835, respectively, and 3.7 for type strain 49188. Western blot an alysis using H. pylori- and H. felis-specific antibodies detected shared an tigenic epitopes between the ureases of H. pylori, H. felis, and O. anthrop i. The apparent molecular mass of the urease enzymes of the O. anthropi iso lates was determined on 6% nondenaturing gels to be approximately 82 kDa. A ntimicrobial susceptibility tests, using the MicroScan method (Dade Interna tional), revealed multidrug resistance for the O. anthropi isolates with su sceptibilities for the antibiotics amikacin, ciprofloxacin, gentamicin, cef operazone, tobramycin, imipenem, and trimethoprim/sulfamethoxazole.