C. Carlo-stella et al., Peripheral blood progenitor cell mobilization in healthy donors receiving recombinant human granulocyte colony-stimulating factor, EXP HEMATOL, 28(2), 2000, pp. 216-224
Objective. We analyzed the incidence of primitive (LTC-IC) and committed (C
FU-mix, BFU-E, CFU-GM) hematopoietic progenitors detected under steady-stat
e renditions and upon progenitor cell mobilization in a cohort of healthy d
onors receiving recombinant human granulocyte colony-stimulating factor (rh
G-CSF),
Materials and methods. Healthy donors (n = 30) of HLA-mismatched or -matche
d stem cell transplants were mobilized with rhG-CSF (8 mu g/Kg body weight
subcutaneously twice daily until completion of leukapheresis:). PBPC collec
tions were started after 4 days of rhG-CSF therapy.
Results. Steady-state incidence of bone marrow LTC-IC, hut not committed pr
ogenitors, significantly correlated with the numbers of mobilized CD34(+) c
ells (r = 0.6,p = 0.004), CFU-GM (r = 0.79,p = 0.0005) and CFC (r = 0.76, p
= 0.001) detected after 4 days of rhG-CSF therapy. Statistically significa
nt correlations Here also found between steady-state blood CFU-GM I and pea
k numbers of CD341 cells (r = 0.68, p = 0.001), numbers of day 4 CD341 cell
s (r = 0.52,p = 0.005), CFU-GM (r = 0.63, p = 0.002), and CFC (r = 0.61,p =
0.003).
Conclusion. Our data show that in normal volunteers baseline marrow LTC-IC
and blood CFU-GM correlate with rhG-CSF-mobilized PBPC, The potential clini
cal relevance of these findings in the identification of poor mobilizers wi
ll be tested in a prospective study, (C) 2000 International Society for Exp
erimental Hematology. Published by Elsevier Science Inc.