V. Zaman et al., Survival of grafted fetal neural cells in kainic acid lesioned CA3 region of adult hippocampus depends upon cell specificity, EXP NEUROL, 161(2), 2000, pp. 535-561
We hypothesize that the degree of graft cell survival within the damaged CN
S correlates with the specificity of donor cells to the region of grafting.
We investigated graft cell survival following transplantation of fetal mic
rografts into the CA3 region of the adult rat hippocampus at a time-point o
f 4 days after an intracerebroventricular administration of kainic acid (KA
). Grafts consisted of 5'-bromodeoxyuridine (BrdU) labeled embryonic day (E
) 19 cells from hippocampal fields CA3 and CA1 and E15 and E19 cells from t
he striatum. Absolute cell survival in these grafts was quantitatively anal
yzed at 1 month postgrafting, using BrdU immunostaining of serial sections
and three-dimensional reconstruction of grafts. Absolute graft cell surviva
l in lesioned CA3 was dramatically greater for cells having hippocampal ori
gin (CA3 cells, 69% cell survival; CA1 cells, 42% cell survival) than those
having nonhippocampal origin, such as striatal cells (E15 cells, 12% cell
survival; E19 cells, 4% cell survival). This difference is in sharp contras
t to survival of these cells in culture, where E19 cells from both hippocam
pal and nonhippocampal origins exhibited similar survival. Comparison of su
rvival among hippocampal cell types indicated significantly greater surviva
l for cells that are specific to the lesioned area (i.e., CA3 cells) than f
or those that are nonspecific to the lesioned area (i.e., CA1 cells). Graft
cell survival in the intact CA3 region (contralateral to KA administration
), however, did not differ either between cells having hippocampal and nonh
ippocampal origins or between CA3 and CA1 cells (CA3 cells, 26% cell surviv
al; CA1 cells, 33% cell survival; and E15 striatal cells, 20% cell survival
). These results underscore the finding that enhanced survival of fetal cel
l, grafts in the lesioned CNS is critically dependent upon the specificity
of donor fetal cells to the region of transplantation. Thus, grafting of ce
lls that are specific to the lesioned area is a prerequisite for achieving
maximal graft cell survival and integration in the lesioned host CNS. (C) 2
000 Academic Press.