M. El-behhari et al., Evidence for a lectin in Kluyveromyces sp that is involved in co-flocculation with Schizosaccharomyces pombe, FEMS MICROB, 184(1), 2000, pp. 41-46
Co-flocculation is the aggregation of yeasts belonging to different genera
or species. Kluyveromyces bulgaricus and Kluyveromyces lactis jc are self-f
locculent, but they can also co-flocculate with the non-flocculent yeast Sc
hizosaccharomyces pombe 972 h(-). This co-flocculation is inhibited by D-ga
lactose and galactose derivatives and involves the binding of a galactose-s
pecific proteinic receptor (or lectin) of Kluyveromyces sp, to the cell wal
l galactomannans of S. pombe. The proteinic receptor is strongly anchored i
n the cell wall, it was partially purified by affinity chromatography using
immobilized S. pombe galactomannans. This galactose-specific proteinic rec
eptor does not appear to interfere in K. bulgaricus or K lactis self-floccu
lation, which is mediated by another galactose-specific lectin weakly linke
d at the cell wall. (C) 2000 Federation of European Microbiological Societi
es. Published by Elsevier Science B.V. Ail rights reserved.