Antioxidative property of T-0970, a new ureidophenol derivative

We investigated the antioxidative property of T-0970, a newly synthesized u reidophenol derivative. The inhibitory effect of T-0970 on spontaneous lipi d peroxidation in rat brain was 10 times greater than those of well-known a ntioxidants such as butylhydroxytoluene (BHT), probucol and alpha-tocophero l. T-0970 also showed dose-dependent free radical scavenging activities in vitro for both superoxide anions and hydroxyl radicals. The radical-scaveng ing potencies of T-0970 were about 10-30 times stronger than those of BHT. We evaluated the in vivo antioxidative ability of T-0970 in the animal mode l of acute oxidative tissue injury in rats. Intraperitoneal injection of fe rric nitrilotriacetate (Fe/NTA) caused an acute and remarkable increase in the level of thiobarbituric acid-reactive substances (TBARS) in both plasma and the liver, and also resulted in a considerable elevation of the plasma levels of GOT and GPT indicative of hepatic injury. Both oral and intraven ous administration of T-0970 dose-dependently depressed these diagnostic pa rameters. These results indicate that T-0970 may have a therapeutic potenti al in various diseases associated with oxidative tissue injury.