Gene structure and chromosome mapping of mouse transcription elongation factor S-II (Tcea1)

We report the organization and chromosome localization of the mouse transcr iption elongation factor S-II gene (Tcea1). This gene was found to be a sin gle copy gene consisting of 10 exons spanning approximately 30 kb. Its orga nization was the same as those of the mouse testis-specific S-II gene (Tcea 2) and Xenopus general S-II gene (xTFIIS.oA), but different from that of th e human S-II gene family. We also identified a processed pseudogene (Tcea1- ps1) with a sequence highly homologous to those of S-II cDNAs but containin g a translation termination codon within its open reading frame. Linkage an alysis showed that Tcea1 and Tcea1-ps1 are mapped on mouse chromosomes 1 an d 15, respectively. Relationships between Tcea1 and S-II cDNAs isolated so far are discussed. (C) 2000 Elsevier Science B.V. All rights reserved.