Exclusion is the recommended method for removing roosting bats from bu
ildings, but is often difficult to accomplish. A simpler way to limit
bat-human conflicts may be to modify new and existing buildings to dis
courage colonies from initially taking up residence. An understanding
of the physical and microclimatic characteristics of maternity roosts
is a prerequisite when modifying buildings to discourage colonies. We
investigated Factors influencing maternity roost selection in big brow
n bats (Eptesicus fuscus) by comparing characteristics of bat-occupied
buildings with bat-unoccupied buildings at 10 sites. Bat-occupied bui
ldings were significantly older, more likely to have galvanized steel
(tin) roofs, more accessible to bats, and taller than randomly selecte
d unoccupied buildings. In paired surveys, occupied attics were signif
icantly more accessible to bats than physically similar unoccupied att
ics and exhibited significantly higher temperatures and wider temperat
ure gradients. Disturbance levels, light levels, and humidity did not
differ between occupied and paired unoccupied attics. To discourage ba
ts from initially establishing a maternity roost within a building, li
mit all access points. In buildings where this is difficult, attics ca
n be made less suitable as roost sites by reducing attic temperatures
during the summer months. Bat boxes intended to house displaced matern
ity colonies should be designed to provide high daily temperatures and
wide temperature gradients.