High-loading supports for oligonucleotide synthesis

A simple protocol for the preparation of high-loading supports, suitable fo r large-scale synthesis of oligonucleotides. has been developed. The method involves the use of inexpensive reagents and is amenable to large-scale pr eparation of supports. The derivatized supports were successfully employed in an automated DNA synthesizer without any difficulty. The quality of the synthesized oligonucleotides was found to be comparable to that or the corr esponding oligomers prepared with commercially available standard supports.