The role of two important tumour suppressor genes, p16 and p53, was evaluat
ed in cartilaginous tumour tissues. Genomic DNA from 22 chondrosarcomas, 5
benign chondroid tumours, 1 sample of reactive proliferative cartilage and
2 samples of normal cartilage were analysed using polymerase chain reaction
, single strand conformational polymorphism, DNA sequencing and methylation
-specific polymerase chain reaction. The p16 gene was found to be partly me
thylated in 5 high-grade chondrosarcomas and homozygously deleted in 1 chon
drosarcoma Moreover, a polymorphism was detected in 3 malignant tumours, bu
t not in benign tumours or normal cartilage. Analysis of the p53 gene revea
led an unchanged structure in all samples. These findings show a role for p
16, but not p53, in chondrosarcoma. (C) Wiley-Liss. Inc.