Desulfotomaculum alkaliphilum sp nov., a new alkaliphilic, moderately thermophilic, sulfate-reducing bacterium

A new moderately thermophilic, alkaliphilic, sulfate-reducing, chemolithohe terotrophic bacterium, strain S1T, was isolated from a mixed cow/pig manure with neutral pH. The bacterium is an obligately anaerobic, non-motile, Gra m-positive, spore-forming curved rod growing within a ph range of 8.0-9.15 (optimal growth at pH 8.6-8.7) and temperature range of 8.0-9.15 (optimal g rowth at pH 8.6-8.7) and temperature range of 30-58 degrees C (optimal grow th at 50-55 degrees C). The optimum NaCl concentration for growth is 0.1 %. Strain S1(T) is an obligately carbonate-dependent allkaliphile. range of T he G+C content of the DNA is 40.9 mol%. A limited number of compounds are u tilized as electron donors,, including H-2+acetate, formate, ethanol, lacta te and pyruvate. Sulfate, sulfite and thiosu8fate, but not sulfur or nitrat e, can be used as electron accepters. Strain S1T is able to utilize acetate or yeast extract as sources of carbon. Analysis of the 16S rDNA sequence a llowed strain S1(T) (= DSM 12257(T)) to be classified as a representative o f a new species of the genus Desulfotomaculum, Desulfotomaculum alkaliphilu m sp. nov.