Assignment of Alteromonas elyakovii KMM 162(T) and five strains isolated from spot-wounded fronds of Laminaria japonica to Pseudoalteromonas elyakovii comb. nov and the extended description of the species

A marine bacterium, Alteromonas elyakovii KMM 162T, which was described rec ently, and five strains isolated from spot-wounded fronds of Laminaria japo nica have been subjected to phylogenetic analysis, and geno- and phenotypic characterization. The phenotypic features of Pseudoalteromonas elyakovii s trains were closely related to that of Pseudoalteromonas espejiana IAM 1264 0(T), but utilization of three carbon compounds (D-mannose, L-tyrosine and trehalose) distinguished both species. The G+C content of Pseudoalteromonas elyakovii was between 38.5 and 38.9 mol%. Pseudoalteromonas elyakovii KMM 162(T) and the five Laminaria isolates constitute a single species differen t from any other Alteromonas and Pseudoalteromonas species as revealed by D NA-DNA hybridization data, especially Pseudoalteromonas distincta KMM 638(T ) (52.4%), Pseudoalteromonas citrea KMM 216 (49.5 %), Pseudoalteromonas car rageenovora NCIMB 302(T) (46.9 %) and Pseudoalteromonas espejiana IAM 12640 T (29.9 %). All the data indicated that Alteromonas elyakovii KMM 162T shou ld be reclassified as Pseudoalteromonas elyakovii and five strains isolated from Laminaria japonica have to be included in the species. Pseudoalteromo nas elyakovii comb. nov. (type strain, KMM 162(T) = ATCC 700519(T)) is prop osed and a set of phenotypic features which differentiate the Pseudoalterom onas species is described.