The efficacy of several organic acids against growth of molds typically fou
nd in animal feed was determined. A plate assay was used in which paper dis
ks soaked in a spore solution were placed on the surface of agar plates con
taining increasing concentrations of the respective organic acid. Mold grow
th radiated from the disks, and the radius was considered indicative of the
degree of inhibition as compared to a control. The efficacy of each of the
organic acids against the various molds varied substantially. Valeric acid
, propionic acid, and butyric acid displayed the highest efficacy against a
ll molds with the effective concentrations ranging from 0.05 to 0.25%. Othe
r acids, including acetic acid, lactic acid, and benzoic acid, required con
centrations of 10 lb/ton or more for effective mold inhibition. Susceptibil
ity of molds to inhibition by organic acids was in the order Fusarium spp.
> Aspergillus spp. > Penicillium spp., with Fusarium spp. being the most su
sceptible mold.