Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase A1 from Bacillus circulans WL-12

Authors
Hashimoto, M Honda, Y Nikaidou, N Fukamizo, T Watanabe, T
Citation
M. Hashimoto et al., Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase A1 from Bacillus circulans WL-12, J BIOSCI BI, 89(1), 2000, pp. 100-102
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
89
Issue
1
Year of publication
2000
Pages
100 - 102
Database
ISI
SICI code
1389-1723(200001)89:1<100:SMOASS>2.0.ZU;2-Y
Abstract
To study the involvement of Asp280 of chitinase Al from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases re vealed that the residue is not absolutely essential for the catalytic react ion and thus, does not act as the catalytic nucleophile.