Prediction of electrophoretic mobilities in non-aqueous capillary electrophoresis - Optimal separation of quinolones in acetonitrile-water media

A model of electrophoretic behaviour is used to predict the optimum conditi ons for the separation of a series of quinolones zwitterionic substances in mixtures of acetonitrile-water up to 30% (w/w) of acetonitrile. The effect of pH, pK(a), the electrophoretic mobility of protonated and anionic speci es, and activity coefficients on the electrophoretic behaviour of quinolone s is considered. The model proposed allows the resolution between substance s in acetonitrile-water mixtures to be predicted from a few experimental da ta and thus permits one to obtain the best experimental conditions for sepa ration methodologies. (C) 2000 Elsevier Science B.V. All rights reserved.