Influence of pH and pK(a) values on electrophoretic behaviour of quinolones in aqueous and hydro-organic media

Citation
D. Barron et al., Influence of pH and pK(a) values on electrophoretic behaviour of quinolones in aqueous and hydro-organic media, J CHROMAT A, 871(1-2), 2000, pp. 381-389
Citations number
43
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
Volume
871
Issue
1-2
Year of publication
2000
Pages
381 - 389
Database
ISI
SICI code
Abstract
Through correct pH measurements, pK(a) and activity coefficient values, a m odel describing their effect on electrophoretic behaviour of substances is established. The suggested model uses the pH values in the acetonitrile-wat er mixtures used and takes into account the effect of activity coefficients . The model permits the calculation of acidity constants of analytes in hyd ro-organic media and also the prediction of the effect of pH on the electro phoretic mobility. The model is tested by determining the dissociation cons tants of a series of nine quinolones in acetonitrile-water mixtures of 0, 5 .5, 10 and 30% (w/w) acetonitrile. (C) 2000 Elsevier Science B.V. All right s reserved.