The alterations of the activities of coagulation inhibitors and fibrinolytic factors in stored cord blood could affect the yield of progenitor cells during processing
Yh. Lee et al., The alterations of the activities of coagulation inhibitors and fibrinolytic factors in stored cord blood could affect the yield of progenitor cells during processing, J HEMATH ST, 8(6), 1999, pp. 653-657
Citations number
14
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
We assessed the changes in the activities of hemostatic variables by the st
orage temperature and time interval between collection and separation of co
rd blood (CB) and analyzed their relationship with the yield of progenitor
cells during processing. Total nucleated cell (TNC) and CD34(+) cell counts
were significantly higher in the CB stored at ambient temperature than at
4 degrees C. The significant loss of TNC and CD34(+) cells continued to 24
h after collection in CB stored at 4 degrees C, but loss of TNC began only
after 24 h at ambient temperature. There were no changes in the plasma acti
vities of antithrombin III (ATIII) and plasminogen. The activity of protein
C was decreased significantly until 24 h after collection, particularly in
CB stored at 4 degrees C. The activity of az-antiplasmin was decreased unt
il 24 h in CB stored at 4 degrees C and from 24 h in CB stored at ambient t
emperature. These data suggest that the alterations in the activities of co
agulation inhibitors and fibrinolytic factors could be an important factor
in coagulability, particularly in CB stored at 4 degrees C compared to ambi
ent temperature, and also affect the yield of progenitor cells in processed
CB.