A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay

Authors
Hallensleben, W Biro, L Sauder, C Hausmann, J Asensio, VC Campbell, IL Staeheli, P
Citation
W. Hallensleben et al., A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay, J IMMUNOL M, 234(1-2), 2000, pp. 149-151
Citations number
1
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGICAL METHODS
ISSN journal
00221759 → ACNP
Volume
234
Issue
1-2
Year of publication
2000
Pages
149 - 151
Database
ISI
SICI code
0022-1759(20000203)234:1-2<149:APITMC>2.0.ZU;2-5
Abstract
The use of multiprobe RPAs is becoming an increasingly popular method for t he detection and quantitation of RNA levels in cells and tissues. Here we r eport that due to a polymorphism in the 3'-noncoding region of the mouse Cr g-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possi bly other mouse strains that may lead to false conclusions regarding expres sion of the Crg-2/IP-10 and MCP-1 genes. (C) 2000 Elsevier Science B.V. All rights reserved.