Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance

Authors
Decker, J Weinberger, K Prohaska, E Hauck, S Kosslinger, C Wolf, H Hengerer, A
Citation
J. Decker et al., Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance, J IMMUNOL M, 233(1-2), 2000, pp. 159-165
Citations number
12
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGICAL METHODS
ISSN journal
00221759 → ACNP
Volume
233
Issue
1-2
Year of publication
2000
Pages
159 - 165
Database
ISI
SICI code
0022-1759(20000113)233:1-2<159:COAHPS>2.0.ZU;2-W
Abstract
We describe the isolation from a large phagemid library of a human pancreat ic secretory trypsin inhibitor (hPSTI) mutant that binds to Legionella pneu mophila. To gain further insight into the binding kinetics of the isolated hPSTI mutant, an immunosensing system based on a quartz crystal microbalanc e (QCM) was used. In contrast to ELISA procedures, k(on) and k(off) rates c ould be derived from the QCM sensograms. Thus, it is possible to characteri ze specific intermolecular interactions between proteins and phages isolate d from large phage display libraries by QCM. (C) 2000 Elsevier Science B.V. All rights reserved.