Generation of a large complex antibody library from multiple donors

Authors
Little, M Welschof, M Braunagel, M Hermes, I Christ, C Keller, A Rohrbach, P Kurschner, T Schmidt, S Kleist, C Terness, P
Citation
M. Little et al., Generation of a large complex antibody library from multiple donors, J IMMUNOL M, 231(1-2), 1999, pp. 3-9
Citations number
10
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGICAL METHODS
ISSN journal
00221759 → ACNP
Volume
231
Issue
1-2
Year of publication
1999
Pages
3 - 9
Database
ISI
SICI code
0022-1759(199912)231:1-2<3:GOALCA>2.0.ZU;2-U
Abstract
We have generated a large complex library of single chain antibodies based on four individual libraries from each of 50 donors. DNA coding for the hea vy and light chain variable domains of the IgM and IgG repertoires was ampl ified by PCR using two different sets of primers. Each individual library w as composed of approximately 1-5 x 10(7) independent clones giving a final combined library of 4 x 10(9) members. Screening this library by phase disp lay of single chain antibodies with small haptens, peptides and proteins yi elded specific antibodies for each class of antigen. (C) 1999 Elsevier Scie nce B.V. All rights reserved.