The preparation, characterisation, and DNA adduct profile of 2-amino-2-methyl-3-butanoneoximedichloroplatinum(II), a platinum(II) complex designed tobind to GpA sequences of DNA

Authors
Ling, ECH Allen, GW Vickery, K Hambley, TW
Citation
Ech. Ling et al., The preparation, characterisation, and DNA adduct profile of 2-amino-2-methyl-3-butanoneoximedichloroplatinum(II), a platinum(II) complex designed tobind to GpA sequences of DNA, J INORG BIO, 78(1), 2000, pp. 55-62
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics","Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF INORGANIC BIOCHEMISTRY
ISSN journal
01620134 → ACNP
Volume
78
Issue
1
Year of publication
2000
Pages
55 - 62
Database
ISI
SICI code
0162-0134(20000115)78:1<55:TPCADA>2.0.ZU;2-P
Abstract
The complex, 2-amino-2-methyl-3-butanoneoximedichloroplatinum (II) [Pt(ambo )Cl-2], was chosen because of its potential to bind to GpA sequences of dup lex DNA. Crystals of [Pt(ambo)Cl-2] are monoclinic, space group, P2(1)/n, a = 6.799(4), b = 17.642(5), c = 8.193(2) Angstrom, beta = 102.10(3)degrees, Z = 4, R = 0.033 (1864 F). The binding of [Pt(ambo)Cl-2] to salmon-sperm D NA was studied using enzymatic digestion and HPLC analysis. [Pt(ambo)Cl-2] was found to form fewer GpG and ApG intrastrand adducts and more monofuncti onal adducts than [Pt(en)Cl-2]. Binding to GpA sequences could not be estab lished, but [Pt(ambo)Cl-2] forms substantially more adducts with adenine th an does [Pt(en)Cl-2]. (C) 2000 Elsevier Science Inc. All rights reserved.