Cellulose-based hydrogels as body water retainers

In this work the possibility of using hydrogels as body water retainers for a therapeutic aid in pathologies such as oedemas of various origins was ex plored. For such a purpose, the material requires a good compatibility and a controlled swelling capacity without altering the body electrolyte homeos tasis. The hydrogel was designed to meet the swelling requirements with the physiological constraints and its biocompatibility was assessed either in vitro or in vivo. Absorption tests were performed in order to define the sw elling behavior by varying the pH and ion content of the external solution. The hydrogel swelling capacity was assessed in the presence of various sol vents, in order to evaluate its absorption capacity in solutions similar to biological fluids. In addition, the capacity of the gel to modify electrol yte homeostasis by adsorbing ions such as calcium, potassium and sodium was tested. In order to assess the gel biocompatibility after contact of the h ydrogel with intestinal cells, arachidonic acid relase was determined. No s ignificant intracellular increase of free arachidonic acid was found in the cells after up to 2 h of contact with the gel. The results suggest that, a s far as brief periods are concerned, the gel does not cause an inflammator y response in intestinal cells. (C) 2000 Kluwer Academic Publishers.