Determination of metoprolol enantiomers in human urine by coupled achiral-chiral chromatography

Citation
Kh. Kim et al., Determination of metoprolol enantiomers in human urine by coupled achiral-chiral chromatography, J PHARM B, 22(2), 2000, pp. 377-384
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN journal
07317085 → ACNP
Volume
22
Issue
2
Year of publication
2000
Pages
377 - 384
Database
ISI
SICI code
0731-7085(200003)22:2<377:DOMEIH>2.0.ZU;2-E
Abstract
Achiral-chiral column switching HPLC assay was developed to allow the separ ation and quantitation of the enantiomers of metoprolol in human urine by m eans of fluorescence detection. Urine samples were prepared by liquid-liqui d extraction, followed by HPLC. The racemic metoprolol and internal standar d were separated from the interfering components in urine and quantified on the silica column, and the enantiomers were determined on a Chiralcel OD c hiral stationary phase. The two columns were connected by a switching valve equipped with a silica trap column. Detection limit was 25 ng/ml for each enantiomer. The intra-day variation ranged between 0.38 and 4.94% in relati on to the measured concentration and the inter-day Variation was 0.15-3.13% . It has been applied to the determination of (R)-(+)-metoprolol and (S)-(- )-metoprolol in urine from healthy volunteers dosed with racemic metoprolol tartrate. (C) 2000 Elsevier Science B.V. All rights reserved.