Fumonisin production by Fusarium moniliforme and F-proliferatum isolated from several agricultural commodities and Japanese soil, and detection of new fumonisins

Twelve Fusarium isolates were obtained from several agricultural commoditie s and soil collected in Japan, corn produced in New Guinea, and grapefruit from an unknown source. Their fumonisin-producing ability was tested on cor n grits and 9 of the 12 isolates produced fumonisins. Since 3 isolates prod uced high levels of fumonisins, the extracts of their cultures were analyze d by LC/MS. The data including MS/MS analyses suggested that the isolate fr om unpolished domestic rice produced monomethyl ester of fumonisin B-1. Fur thermore, we detected novel fumonisin B1 analogues in the extract from the grapefruit, although we could not precisely determine their structures.